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-Abstract
The genetic identity of Fasciola species
in Egypt
has been investigated by carrying out the sequence analysis of the ITS-2 region
of rDNA gene. Also, highly repetitive sequences of genomic DNA (124 bp) were
determined and compared. Three isolates of Fasciola recovered from
Egyptian local breeds (sheep, Buffaloes and Cows) were used. Sequence
comparison including ITS-2 sequences of isolates from this study and reference
sequences from Fasciola hepatica and F. gigantica and intermediate
Fasciola in Genbank was done. The results revealed several variable
sites encountered among the investigated isolates in comparison with species of
Fasciola submitted in Genebank. The relationship between Egyptian Fasciola
and Fasciola spp. from different countries were discussed.
DOI
10.21608/eajbse.2014.14500
Keywords
Fasciola spp. Egypt, ITS-2, Repetitive, DNA fragments, Sequencing
Authors
Affiliation
Department of Zoology, Faculty of Science, Ain Shams University, Cairo, Egypt
Email
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https://eajbse.journals.ekb.eg/article_14500.html
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https://eajbse.journals.ekb.eg/service?article_code=14500
Publication Title
Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology
Publication Link
https://eajbse.journals.ekb.eg/
MainTitle
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