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Molecular Characterization of Fasciola spp. in Egypt on the Basis of certain rDNA fragments and Highly Repetitive DNA Sequences

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Last updated: 03 Jan 2025

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Abstract

The genetic identity of Fasciola species
in Egypt
has been investigated by carrying out the sequence analysis of the ITS-2 region
of rDNA gene. Also, highly repetitive sequences of genomic DNA (124 bp) were
determined and compared. Three isolates of Fasciola recovered from
Egyptian local breeds (sheep, Buffaloes and Cows) were used. Sequence
comparison including ITS-2 sequences of isolates from this study and reference
sequences from Fasciola hepatica and F. gigantica and intermediate
Fasciola in Genbank was done. The results revealed several variable
sites encountered among the investigated isolates in comparison with species of
Fasciola submitted in Genebank. The relationship between Egyptian Fasciola
and Fasciola spp. from different countries were discussed.

DOI

10.21608/eajbse.2014.14500

Keywords

Fasciola spp. Egypt, ITS-2, Repetitive, DNA fragments, Sequencing

Authors

First Name

Hoda

Last Name

Taha

MiddleName

A.

Affiliation

Department of Zoology, Faculty of Science, Ain Shams University, Cairo, Egypt

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Volume

6

Article Issue

1

Related Issue

2864

Issue Date

2014-12-01

Receive Date

2018-09-24

Publish Date

2014-12-01

Page Start

19

Page End

28

Print ISSN

2090-0783

Online ISSN

2090-0856

Article File

EAJBSE_Volume 6_Issue 1_Pages 19-28.pdf

PDF . 316.9kB

Link

https://eajbse.journals.ekb.eg/article_14500.html

Detail API

https://eajbse.journals.ekb.eg/service?article_code=14500

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3

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Original Article

Type Code

687

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology

Publication Link

https://eajbse.journals.ekb.eg/

MainTitle

Molecular Characterization of Fasciola spp. in Egypt on the Basis of certain rDNA fragments and Highly Repetitive DNA Sequences

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Article

Created At

22 Jan 2023