The silkworm, Bombyx mori being a monophasic insect, derives all nutrients required for its development from mulberry leaves itself. Silkworm feeding is one of the most important major areas of research in sericulture. The distinctive characteristics of nanomaterials have the ability to enhance and development of various sectors including agriculture, industry, cosmetics and medicine. Silver nanoparticles (AgNPs) it is one of the most common nanoparticles that are known as a noble metal, a widely used antibacterial material, and non-antibiotic, and have demonstrated toxic and other potentially adverse effects on mammals. However, the deleterious effects of Silver nanoparticles on insects are still unknown. Here, we researched the effects of silver nanoparticles on the model invertebrate organism Silkworm, Bombyx mori L.
The aim of this study was to evaluate the effects of ingesting Silver nanoparticles through food to assess their toxicity effects on the silkworms, After treatment with various concentrations (5,10,50 and 100 ppm), of silkworm and midgut histopathological analysis. Our research showed abnormalities histopathological of midgut-damaged epithelium cells detached from the broken basement membrane, some cells become thicken and large size than control in some areas and the space between the peritrophic membrane   layers and   the epithelial layer, emptied them 
cytoplasmic contents.