In the present study, we investigated the potential protective effects of royal jelly against doxorubicin-induced nephrotoxicity in Ehrlich ascites tumor bearing mice. Adult male albino mice were randomly divided into eight groups: the control, royal jelly, doxorubicin, and royal jelly plus doxorubicin groups. Biochemical, oxidative stress, and histopathological and immunohistochemical methods were utilized for evaluation of the reno-toxicity. Blood was collected and analyzed for blood urea nitrogen (BUN), uric acid and creatinine. The renal samples were stored for the measurement of malondialdehyde (MDA), glutathione peroxidase (GSH) and superoxide dismutase (SOD) activities and processed for histopathological examinations. Administration  of  doxorubicin  to  mice bearing Ehrlich tumor  induced  a marked renal dysfunction, characterized with a significant increase in serum BUN, uric acid and creatinine concentrations, and they had higher renal MDA and decreased in GSH and SOD concentrations. In the groups that were feed on RJ in association with DOX, improvement was observed in some oxidative stress parameters and certain other biochemical, histopathological and immunohistochemical parameters. The royal jelly exerted significant protection against renal damage induced by doxorubicin through reduction of the elevated activities of serum renal functions. Moreover, royal jelly blocked doxorubicin-induced lipid peroxidation through decreasing the malondialdehyde formation. In conclusion, royal jelly has a capability to attenuate doxorubicin-induced nephrotoxicity.