The objective of the present study was to investigate the effective of Titanium dioxide nanoparticles as acause of gross malformation and histopathological changes in albino mice fetal brain ,40 pregnant albino mice were divided into 4 equal groups then Titanium dioxide nanoparticles was intraperitonialinjected with different concentration (50,100,150,200) mg/kg body weight. On day 18^th of gestation, pregnant mice was anaesthetizing, uterine horns were removed ,Then the Embryo toxicity was assessed by study the gross malformation and histopathological examination for fetus brain. The results showed that malformation represented as a cleft lip, cleft palate, malformation of face  exophthalmia eye, lose of external ear, bending of the hind limbs, shortening of the fore limbs, the tail malformation, angulations of the trunk, exocephalius and wrinkled congested skin. Microscopically histological lesions of fetus brain shows an infiltration of inflammatory cells, congestion, necrosis and vocal degeneration.