Nuweiba city, South Sinai, Egypt is one of the poorly-studied areas in terms of the presence of medically-important dipteran species. Therefore, in the current study a survey of flies (order: Diptera) of medical importance in Nuweiba city was carried out during winter and summer of 2019. Six traps baited with two sources of attractants – decayed fish pieces and a ready-made botanical extract – were used to collect the flies in four selected localities. A total of 374 individuals belonging to 11 species within 9 genera under 6 families (Culicidae, Ephydridae, Ulidiidae, Calliphoridae, Muscidae, and Sarcophagidae) were captured, and the identification key was provided. The highest numbers of adult flies were found in summer in “Habiba organic farm" using traps baited with decayed fish pieces (n=159). Two species (Musca domestica L. and Culex pipiens L.) were the most predominant species with a total of 141 and 100 captured specimens, respectively. The collected specimens included medically important species, as disease vectors and myiasis producers in humans and animals, which belong to three families (Calliphoridae, Muscidae, and Sarcophagidae); besides the two mosquito species (Culex pipiens and Culiseta longiareolata Macquart) that are known as vectors for many diseases of humans. In addition, Sarcophaga (Liopygia) surcoufi is newly recorded in Sinai. In conclusion, the aforementioned dipterous species may have the potential of transmitting diseases to the community dwellers in Nuweiba city; therefore, they must be controlled.