Among Oniscidea suborder, Porcellionidae is the richest and widely-distributed Family. However, the phylogeny investigations of its taxa were not completely covered. Therefore, a phylogeographic population and genetic diversity study was carried out here on two cosmopolitan terrestrialisopod species(Porcellionides pruinosus and Porcellio laevis) by utilizing mitochondrial cytochrome c oxidase subunit 1 (CO1) gene. The sequencing of CO1 among the understudied populations showed nucleotide length ranged from 646 base pairs (bp) to 660 bp. The average contents of nucleotide bases A+T in Porcellionides pruinosus and Porcellio laevis were 63.8% and 63.3%, respectively. Also, the average content of pyrimidines (C+T) bases in Porcellionides pruinosus and Porcellio laevis was higher than the purines content. Nucleotide bases A+T contents were higher than nucleotide bases C+G contents in all populations. The pairwise genetic distances among the understudied Porcellionides pruinosus populations ranged from 0.000 to 0.002. The most related populations were in Qeft and Deshna, where the genetic distance was 0.000. The pairwise genetic distances among the understudied populations of Porcellio laevis ranged from 0.017 to 0.033. The most related populations were in Qeft and Hurghada, where the genetic distance was 0.017. Furthermore, Porcellio laevis populations need more investigations, as it seems to be polyphyletic comparing to Porcellionides pruinosus populations.