The present study aims to evaluate the role of ginger extract against the ovotoxic and/or carcinogenic effects induced by 7, 12-dimethylbenz[a]anthracene (DMBA) in the ovarian tissues of female albino rats. Ginger (Zingiber officinale) has acquired considerable attention as a botanical dietary due to its active biological constituents of phenolic substances ([6]-gingerol and [6]-paradol) that possess anti-inflammatory, anti-oxidative properties and exerts substantial anti-carcinogenic activities. In the present study, a single dose of 40 mg/kg of 7,12-dimethylbenz[a]anthracene was used to induce ovotoxicity, and aqueous extract of ginger concentration (120 mg/ml) every other day as a possible protective substance. The ultrastructural observations of DMBA-treated group showed some differences such as fibroblast cells with condensed chromatin, primordial follicles area exhibiting scattered cytoplasmic organelles and ruptured-plasma membrane, some granulosa of the secondary follicles showing cytoplasmic vacuolization and some others revealing multi-nucleated. The ultrastructural observations of combined DMBA and ginger treated rats revealed more or less normal fibroblasts, primordial follicular cells, granulosa of secondary follicles and improvement in the number and shape of mitochondria. Concerning the immunohistochemical observations, the ovary of DMBA treated rats showed a significant increase in the proliferating cell nuclear antigen labeling index (PCNA LI, P ≤ 0.001) with respect to control and/or ginger treated rats. However, DMBA and ginger treated rats showed a significant increase in the PCNA LI (P ≤ 0.01) with respect to control and/or ginger treated rats and a significant decrease (P≤ 0.001) with those of DMBA treated rats. In conclusion, ginger may play a possible role as a protective agent against the ovotoxicity and/or carcinogenicity of DMBA showing an improvement of the ovarian tissue.