Hepatitis C virus infection represents a serious epidemic problem in Egypt, it has the highest presence of the infection globally. Liver cirrhosis and hepatocellular carcinoma is mainly resulted from chronic infection with hepatitis C virus. Many direct antiviral agents have been utilized for treatment, but the hepatotoxicity of those drugs still rarely recorded. The current study was conducted to evaluate the induced toxicity by sofosbuvir and ribavirin. Thirty two female albino rats were divided into four equal groups; group one included rats that were administered orally with sofosbuvir (0.01 mg/kg body weight/day) suspended in distilled water. Group two included rats that were administered orally with ribavirin (0.0227 mg/kg body weight/day) suspended in distilled water. Group three included rats that were administered with a combination of sofosbuvir and ribavirin (0.01 and 0.023 mg/kg body weight/day, respectively) suspended in distilled water. Group 4 consisted of untreated animals and were served as control group. The present study found a significant decrease in mean corpuscular volume and mean corpuscular haemoglobin values and a significant elevation of red blood corpuscles distribution width percentage in groups treated with ribavirin. Moreover, a significant elevation of aminotransferases, alkaline phosphatase, ammonia, total bilirubin and malondialdehyde were found in the treated groups as well. Sofosbuvir plus ribavirin can induce some hepatotoxicity in normal rats characterized by elevations in aminotransferases and microcytic hypochromic anaemia.