The present study aimed to clarifying the effective and protective role of green tea (GT) against hepatotoxicity and oxidative damage induced by ketoconazol (KT) in male mice. Animals were divided into 6 groups, served as control (C), vehicle treated, KT treated, KT+GT treated, recovery (R) and R+ GT treated. Detection of cytochrome P450 (CYP1A1) was carried out by western blot, in addition to histopathological and biochemical studies. KT administration significant stimulated CYP1A1 in liver compared to control. Sever degenerative and histopathological changes in different treatments was also observed. In addition KT administration induces a significant elevation of lipid peroxidation (LPO), carbonyl protein (CP), total peroxide (TP), Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and Lactate dehydrogenase (LDH) activities in blood plasma. Moreover, significant inhibition of hepatic superoxide dismutase (SOD), catalase (CAT), and depletion of hepatic reduced glutathione (GSH) were observed in different treatments after KT administration. The results of the present study showed that GT normalized hepatic levels of CYP1A1, LPO, CP, TP, CAT, SOD and GSH. The use of green tea protects the liver and reduces the destruction and inflammatory effects resulting from exposing to KT.