The current study was designed to evaluate the efficacy of the male chemosterilant alpha-chlorohydrin (ACH) in the control of three Egyptian wild rodent pests (Order: Rodentia, Family: Muridae), namely; the Cairo spiny mouse, Acomys cahirinus (Desmarest, 1819) the lesser Egyptian gerbil, Gerbillus gerbillus (Olivier, 1801) and Shaw's jird, Meriones shawi Rozet, 1833. The effect of ACH was tested by the examination of the relative weights of the reproductive organs, the sperm parameters, as well as the histological alterations in the testis and epididymis. The results of this study showed that ACH has no significant antifertility effect when given to A. cahirinus at doses of 50 mg/kg body weight for 5 days, 70 mg/kg for 4 days; to G. gerbillus at doses of 50 mg/kg for 3 days, and 60 mg/kg for 4 days; and to M. shawi at doses of 50 mg/kg for 5 days, and 80 mg/kg for 3 days. Taken together, it could be concluded that ACH has a species-specific action, and is not recommended to be used in the control of the rodent pests of the present study.