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146344

Phenotypes of B-Lymphocytes as a Predictor of Steroid Response in Idiopathic Nephrotic Syndrome.

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

Glomerular disorders, HUS, SLE, and other vasculitides.

Abstract

Introduction: The pathogenesis of Idiopathic nephrotic syndrome (INS) is not clear, where the role of B-cells is not yet illustrated.   Aim of the study: To evaluate B- cells subsets as a predictor of steroid therapy sensitivity among INS at initial onset.   Methods: 45 children with idiopathic nephrotic syndrome were recruited in a follow- up study, patients were divided into steroid-sensitive nephrotic syndrome (SSNS) and steroid-resistant nephrotic syndrome (SRNS) groups based on their sensitivities to steroids. Venous blood samples were drawn from all children for assessment of memory B- cell population before and after one year of steroid therapy. Results: We found statistically significant relation between steroid response and total memory CD19+ CD27+B cell at disease onset where it was higher in SSNS group. Our results showed  The best cut-off of total CD19+ B cells in predicting steroid response was ≥32.7 with an area under curve (AUC) of 0.445, a sensitivity of 43.9%, a specificity of 50%, a positive predictive value (PPV) of 90%, a negative predictive value (NPV) of 8% and an accuracy of 44.4% (p>0.05)  We also found that The best cut-off of CD19+ CD 27+ total memory B cells in predicting steroid response was ≥32 with an AUC of 0.817, a sensitivity of 78%, a specificity of 75%, a PPV of 97%, a NPV of  25% and an accuracy of 77.8% (p < 0.05).  Conclusion: CD19+ CD 27+ total memory B cells could help in predicting steroid response among INS patients with a cut-off of total CD 19+B cells above 32.7  and PPV of 90% , a cut-off above 32 in CD19+ CD +27 total memory cells and PPV 97%.  

DOI

10.21608/geget.2021.49509.1016

Keywords

nephrotic syndrome, B-lymphocyte, children, Flow cytometry, Steroid

Authors

First Name

Faika

Last Name

Arab

MiddleName

-

Affiliation

Department of Pediatrics, Pediatric Nephrology Unit, Faculty of Medicine, Zagazig University, Zagazig, Egypt.

Email

faikaarab84@gmail.com

City

zagazig

Orcid

-

First Name

Doaa

Last Name

Youssef

MiddleName

-

Affiliation

Department of Pediatrics, Pediatric Nephrology Unit, Faculty of Medicine, Zagazig University, Zagazig, Egypt.

Email

dody5176@yahoo.com

City

-

Orcid

0000-0001-9948-5690

First Name

Fatma

Last Name

Ibrahim

MiddleName

Alzahraa G.

Affiliation

Department of Pediatrics, Pediatric Nephrology Unit, Faculty of Medicine, Zagazig University, Zagazig, Egypt.

Email

fatma111969@yahoo.com

City

-

Orcid

-

First Name

Lobna

Last Name

El-korashi

MiddleName

A.

Affiliation

Department of Medical Microbiology & Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.

Email

lobna23@yahoo.com

City

Zagazig

Orcid

-

First Name

Hoda

Last Name

Fathey

MiddleName

-

Affiliation

Department of Clinical Pathology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.

Email

hodamoh@gmail.com

City

-

Orcid

-

Volume

15

Article Issue

2

Related Issue

20384

Issue Date

2020-12-01

Receive Date

2020-11-11

Publish Date

2020-12-01

Page Start

28

Page End

35

Print ISSN

1687-613X

Online ISSN

2636-3666

Link

https://geget.journals.ekb.eg/article_146344.html

Detail API

https://geget.journals.ekb.eg/service?article_code=146344

Order

3

Type

Original Article

Type Code

675

Publication Type

Journal

Publication Title

GEGET

Publication Link

https://geget.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023