Background: Helicobacter Pylori (H. Pylori) is a known cause of gastritis and peptic ulcer in children especially among immune compromised patients as those with nephrotic syndrome (NS). The infection is acquired via droplet or fecal-oral routes. Eradication of the organism prevents the development of peptic ulcer disease. Objectives: Assessment of the prevalence of H. Pylori infection by various diagnosis modalities among nephrotics patients. Also, to evaluate the effect of specific treatment on clinical and laboratory findings. Methods: This study was conducted at the Pediatric Nephrology Clinical of Ain Shams University Children′s Hospital.  It included 50 patients with subdivided according to response to steroid therapy into dependent, resistant and responsive in remission. They were 34 males and 16 females with a mean age of 9.5 ± 7.2 years, in addition to 24 healthy children as control group. H. Pylori infection was detected by several methods including serum anti H. Pylori IgA antibodies, Urease test, culture and PCR of a gastric aspirate. Patients who proved positive by one of the last three methods were given triple therapy (amoxicillin, omeprazole and clarithromycin) for one week and then reevaluated after one month by the same tests. Results: Abdominal complaints were present in 27 out of 50 patients (54%). Serum specific IgA was positive in 22 patients (44%). Urease test was positive in 14 patients (28%) while culture for H. Pylori revealed positive results in only two patients (4%). PCR for H. Pylori was positive in 10 patients (20%). Thus, the most sensitive method was serum specific IgA (80%) while the most specific method was culture (100%) then urease test (75%). After treatment, both IgA and PCR positively were significantly reduced and urease positive patients turned negative. Conclusions: Culture of gastric aspirate proved to be the most specific of the methods used while serum specific IgA was the most sensitive compared to PCR of gastric aspirate as a standard test for detection of H. Pylori infection. Triple therapy was very effective in eradicating H. Pylori infection and improving abdominal complaints in children with NS.