Background Urinary Tract Infection is one of the most common bacterial infections among children.. It is noted that the risk of renal damage from Urinary Tract Infection is greatest in children younger than 5 years, thus early diagnosis and prompt treatment are important.   Aim of Work The aim of this study was to assess the prevalence of urinary tract infection in children attending Pediatric outpatient clinic in Zagazig University Children's Hospital. Also it aimed to determine, related risk factors, isolate the organisms that cause Urinary Tract Infection in children and antibiotics susceptibility patterns   Patients and Methods This Cross sectional descriptive study, was conducted on 600 children, (377 males and 223 females) from 2 to 7 years old attending to pediatric outpatient clinic Zagazig University Children's Hospital, All patient groups were subjected to full medical history, physical examination, Dipstick analysis by using both nitrite and leukocyte esterase detector, Microscopic examinations and urine culture for positive cases.   Results The prevalence of Urinary Tract Infection among children included in the current study was (7%). Leukocyte esterase positive were 56 (9.3%), Nitrite positive were 47 (7.8%) and both Leukocyte esterase and Nitrite positive were 17 (2.8%).   Conclusion  The prevalence of Urinary Tract Infection in our study was 7%. Escherichia Coli was the most common organism. Cefotaxime and Amikacin were the most common antibiotic sensitive to the isolates.