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249382

Characterization of Phenoloxidase in The Red Palm Weevil; Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) (Olivier) Using Catechol Substrate

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Last updated: 03 Jan 2025

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Abstract

Phenoloxidases (EC.1.14.18.1) are implicated in the immunity of insects toward microorganisms, so oxidative enzymes such as phenoloxidases (PO) from the 7th instar larvae of the red palm weevil; Rhynchophorus ferrugineus (Oliv.) was partially isolated to characterize its activity using catechol as a substrate. The mitochondrial fraction of larval homogenate was used as enzyme source for partial kinetic studies.Enzyme catalysis was directly proportional to enzyme crude protein conc. up to 200 ug contained in 1 ml of the reaction mixture. Michaelis-Menten kinetics of PO activity was evaluated by constructing Lineweaver Burk double reciprocal plot. Michaelis constant (Km) was, 3.45 X10-4 M and maximum velocity (V max) of PO reaction was 344.8 O.D.min-1 mg protein-1. Effect of pH, temperature and substrate concentration on enzyme reaction was tested. Generally, each variable was chosen while other conditions were at optimum found primarily. Reaction rate was optimal at 10-1 N catechol, neutral medium (pH 7) and 40 C. PO kept its most activity when incubated at 60 C for 15 min before reaction initiation. Phenoloxidases efficiently catalyze catechol. Results in the present study may probably offer a fundamental anchor for future alternative strategies in controlling R. ferrugineus via obstructing its innate defence mechanisms.

DOI

10.21608/eajbsc.2022.249382

Keywords

Phenoloxidases, Red palm weevil, Rhynchophorus ferrugineus (Olivier), Michaelis-Menten kinetics

Authors

First Name

Tarek

Last Name

Amin

MiddleName

R.

Affiliation

Department of Pest Physiology, Plant Protection Research Institute (PPRI), Agricultural Research Center (ARC), Dokki, Giza, Egypt.

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Orcid

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First Name

Nedal

Last Name

Fahmy

MiddleName

M.

Affiliation

-Department of Pest Physiology, Plant Protection Research Institute (PPRI), Agricultural Research Center (ARC), Dokki, Giza, Egypt -Department of Biology, University Collage of Taymaa, University of Tabouk (ut), Kingdom of Saudi Areabia(Ksa)

Email

nedalashraf33@hotmail.com

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Volume

14

Article Issue

1

Related Issue

29927

Issue Date

2022-06-01

Receive Date

2022-05-05

Publish Date

2022-06-28

Page Start

499

Page End

510

Print ISSN

2090-0767

Online ISSN

2090-083X

Article File

EAJBSC_Volume 14_Issue 1_Pages 499-510.pdf

PDF . 733.1kB

Link

https://eajbsc.journals.ekb.eg/article_249382.html

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https://eajbsc.journals.ekb.eg/service?article_code=249382

Order

42

Type

Original Article

Type Code

673

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

Publication Link

https://eajbsc.journals.ekb.eg/

MainTitle

Characterization of Phenoloxidase in The Red Palm Weevil; Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) (Olivier) Using Catechol Substrate

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Article

Created At

22 Jan 2023