This study aims to explore the larvicidal potency of zinc oxide nanoparticles (ZnONPs) against house fly (Musca domestica) larvae. Twenty-five third instar larvae were incubated with larval medium and different serial concentrations of ZnONPs.  For each concentration, four replicates were concerned for a total of 100 larvae. A set of the control group using distilled water was included. The number of surviving larvae was counted after 24 hours of incubation. The average larval mortality data were subjected to Probit analysis for calculating LC10, LC50 and LC90. Subsequently, the tissue homogenates of larvae treated with LC10, LC50 and LC 90 of ZnONPs were involved in different cellular and biochemical assays regarding superoxide dismutase, catalase, nitric oxide, lipid peroxidase and glutathione reductase enzymes as indicators of oxidative stress and cellular damages and also for detecting the levels of total protein and albumin as indicators of cellular functions. Results revealed that LC10, LC50 and LC90 were 0.08, 1.00 and 12.9 mg/ ml respectively. Levels of total protein displayed a significant increase while levels of albumin revealed a significant decrease. A significant decreasing pattern was observed in levels of oxidative stress enzymes among different larval groups including superoxide dismutase, catalase enzyme and glutathione reductase. On the other hand, levels of cellular damage enzymes showed a significant increase in lipid peroxidase enzyme and nitric oxide levels. In conclusion, the results of this study showed that ZnONPs exhibit larvicidal potency against house flies (Musca domestica) decreasing the activities of oxidative stress enzymes and increasing the levels of cellular damage.