The contamination of raw milk by S. aureus remains an important issue in dairy food production. The objectives of this study were to determine the prevalence and antimicrobial resistance of Staphylococcus aureus isolated from raw cow milk in the Northwest of Algeria. A total of 81 samples of raw milk were collected from (shops and farms.  S. aureus was detected in 15 samples (18,5%). 25 S. aureus strains were identified of which 5 were positive for methicillin-resistant S. aureus and 9 were multiple antibiotic resistance (MAR). The S. aureus isolates showed resistance to penicillin G (92%), followed by tetracycline (72%), ofloxacin (24%), cefoxitin (24%), oxacillin (20%), Sulfamethoxazole/trimethoprim (8%) lincomycin, Tobramycin, Lincomycin, Fosfomycin and Fusidic acid (4%). the MIC determination against OXA of these strains varied from 35 to 190 (μg/mL). The results indicated that raw cow milk samples contaminated by S. aureus can be a potential risk to public health.