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206175

Isolation, Purification and Identification of CFP29 from Mycobacterium tuberculosis H37Rv culture filtrate proteins

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Last updated: 03 Jan 2025

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Abstract

Tuberculosis (TB) continues to be a serious worldwide health issue. Human tuberculosis (TB) is the commonest cause of mortality from one infectious agent, with eight million new cases and two million fatalities each year. In many animal models of TB, proteins isolated from the culture filtrate of Mycobacterium tuberculosis promote protective immunity. The extracellular proteins of Mycobacterium tuberculosis were isolated, purified and identifiedin the current study. M. tuberculosis H37Rv was culturedin Souton's medium and the extracellular proteins were isolated employing an ion-exchange column chromatography before their purification and characterization using SDS- PAGE, western blotting and N- terminal sequencing. CFP29 was identified and purified in M. tuberculosis H37Rv culture filtrate. However, further characterization of this protein is needed to be utilized as an effective T-cell antigen produced from culture filtrates. 

DOI

10.21608/eajbsc.2021.206175

Keywords

isolation, Purification, CFP29, Mycobacterium tuberculosis, culture filtrate proteins

Authors

First Name

Mansoor

Last Name

Alsahag

MiddleName

-

Affiliation

Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Albaha University, Albaha, Saudi Arabia

Email

maleshaq@bu.edu.sa

City

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Orcid

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Volume

13

Article Issue

2

Related Issue

26732

Issue Date

2021-12-01

Receive Date

2021-10-05

Publish Date

2021-12-01

Page Start

189

Page End

198

Print ISSN

2090-0767

Online ISSN

2090-083X

Article File

EAJBSC_Volume 13_Issue 2_Pages 189-198.pdf

PDF . 601.0kB

Link

https://eajbsc.journals.ekb.eg/article_206175.html

Detail API

https://eajbsc.journals.ekb.eg/service?article_code=206175

Order

14

Type

Original Article

Type Code

673

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

Publication Link

https://eajbsc.journals.ekb.eg/

MainTitle

Isolation, Purification and Identification of CFP29 from Mycobacterium tuberculosis H37Rv culture filtrate proteins

Details

Type

Article

Created At

22 Jan 2023