The aim of the current investigation has been to evaluate the toxic effects of Diflubenzuron (DFB) insecticide on domestic rabbits. In this study, we aimed to evaluate the toxicity of DFB on some reproductive parameters in male Oryctolagus cuniculus rabbits. In animals (6 to 7 months and 1700 ± 170 g) the insecticide was used on three different doses:250,500,750 mg/kg. The insecticide was daily administrated for four weeks of experimental periods. Results showed the following effects as compared with the control group: a-decrease in reproductive indicators (concentration, motility, speed, and vitality of spermatozoa) and in serum testosterone concentration. Histological examination showed pathological alterations such as a remarkable reduction in spermatozoa concentration and cellular malformation within seminiferous tubules and epithelial cells especially in groups treated by the highest dose of Diflubenzuron, data demonstrated that DFB caused an effect on reproductive function in adult male rabbits.