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Assessment of Genotoxicity of Potassium Nitrate and Sodium Benzoate in Drosophila melanogaster Using Smart and Comet Assays

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Last updated: 03 Jan 2025

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Abstract

Modern food industry increasingly relies on food additives. The dramatic increase in the demand on preserved ready to eat food products making up to 75 percent of the diet of western society lead to neglectance of the harmful effects of the food additives on human health; among these are hypersensitivity, allergic reactions, genotoxicity, mutagenicity and more. This proposal investigates genotoxic effects of two commonly used food preservatives; sodium benzoate and potassium nitrate using the somatic mutation and recombination test (SMART) and comet assay. Two important end points of genotoxicity can be covered by in vivo assay systems; comet assay detects DNA damage, while the Drosophila transgenic animal model recognizes gene mutation and/or chromosomal aberrations. Both of the tested compounds showed significantly high levels of tumor induction and frequency compared to a negative control in SMART assay accompanied with a significant amount of DNA damage detected by the comet assay indicating their high potential of being genotoxic materials

DOI

10.21608/eajbsc.2018.22715

Keywords

Genotoxic, comet, SMART, Drosophila, food additives

Authors

First Name

Amal

Last Name

Aledwany

MiddleName

Z.

Affiliation

Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt

Email

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Orcid

-

First Name

Wesam

Last Name

Basal

MiddleName

T.

Affiliation

Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt

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-

Orcid

-

First Name

Neima

Last Name

Al-Senosy

MiddleName

K.

Affiliation

Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

Email

neimakotb@yahoo.com

City

-

Orcid

-

First Name

Aliaa

Last Name

Issa

MiddleName

M.

Affiliation

Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt

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-

City

-

Orcid

-

Volume

10

Article Issue

2

Related Issue

4296

Issue Date

2018-12-01

Receive Date

2018-12-24

Publish Date

2018-12-01

Page Start

83

Page End

97

Print ISSN

2090-0767

Online ISSN

2090-083X

Article File

EAJBSC_Volume 10_Issue 2_Pages 83-97.pdf

PDF . 768.3kB

Link

https://eajbsc.journals.ekb.eg/article_22715.html

Detail API

https://eajbsc.journals.ekb.eg/service?article_code=22715

Order

7

Type

Original Article

Type Code

673

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

Publication Link

https://eajbsc.journals.ekb.eg/

MainTitle

Assessment of Genotoxicity of Potassium Nitrate and Sodium Benzoate in Drosophila melanogaster Using Smart and Comet Assays

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Article

Created At

22 Jan 2023