Subjects
-Tags
-Abstract
Modern food industry increasingly relies on food additives. The dramatic increase in the demand on preserved ready to eat food products making up to 75 percent of the diet of western society lead to neglectance of the harmful effects of the food additives on human health; among these are hypersensitivity, allergic reactions, genotoxicity, mutagenicity and more. This proposal investigates genotoxic effects of two commonly used food preservatives; sodium benzoate and potassium nitrate using the somatic mutation and recombination test (SMART) and comet assay. Two important end points of genotoxicity can be covered by in vivo assay systems; comet assay detects DNA damage, while the Drosophila transgenic animal model recognizes gene mutation and/or chromosomal aberrations. Both of the tested compounds showed significantly high levels of tumor induction and frequency compared to a negative control in SMART assay accompanied with a significant amount of DNA damage detected by the comet assay indicating their high potential of being genotoxic materials
DOI
10.21608/eajbsc.2018.22715
Keywords
Genotoxic, comet, SMART, Drosophila, food additives
Authors
Affiliation
Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt
Email
-City
-Orcid
-Affiliation
Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt
Email
-City
-Orcid
-Affiliation
Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
City
-Orcid
-Affiliation
Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt
Email
-City
-Orcid
-Link
https://eajbsc.journals.ekb.eg/article_22715.html
Detail API
https://eajbsc.journals.ekb.eg/service?article_code=22715
Publication Title
Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Publication Link
https://eajbsc.journals.ekb.eg/
MainTitle
-