Direct-PCR is not common for Y chromosome in forensic laboratories in Egypt. The current investigation was carried out to evaluate the priority of direct PCR technique in Y-STR test over routine extraction method.Results showed that direct PCR over hair samples obtained from five persons who do not use any Hair waxes recorded higher scale of peak heights than those subjected to a prior-PCR DNA extraction. In addition, much better results for direct PCR were obtained when one hair was subjected to direct PCR amplification than using three hairs. This technique may solve the problems of rapid and cost-reducing genotyping of forensic samples, as well as may improve the fluorescence detection of some genetic loci.