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16301

First comparative phenetic studies of the polymorphic species of Coccinella undecimpunctata Linnaeus, using morphometric and RAPD approaches in Egypt

Article

Last updated: 22 Jan 2023

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Abstract

In this study, numerical phenetic method and RAPD-PCR technique were carried out on the species Coccinella undecimpunctata L. and its 13 aberrations which were collected from 10 localities in Egypt. The morphometric analysis and phenotypic features were determined for the aberrations to reveal the phenetic relationships among them. The resulted phenogram showed high degree of variations and affinities (Similarity coeffeciant ranged from 44.7 to 85.7%). RAPD fingerprint profiles were generated by using 5 random primers on genomic DNA to evaluate their phenetic relationships and to investigate the molecular markers among the aberrations genotypes. The similarity coefficient of the produced DNA fragments ranged from 40% to 93.75%. Cluster analysis based on both morphometric and RAPD data showed that the 14 morphs are grouped into 9 clusters against 8 clusters respectively. In addition, PCA plot allowed differentiating three groups from morphometric data against four groups from RAPD data.  

DOI

10.21608/eajbsc.2009.16301

Keywords

Phenetic taxonomy, Morphometric measures, RAPD-PCR, DNA Polymorphism, molecular markers, Aberrations, Coccinella undecimpunctata, Egypt

Authors

First Name

Haitham

Last Name

Badrawy

MiddleName

B. M.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Abbasia- Cairo, Egypt

Email

haithambadrawy@yahoo.com

City

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Orcid

-

First Name

Dalia

Last Name

Salem

MiddleName

A. M.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Abbasia- Cairo, Egypt

Email

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City

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Orcid

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First Name

Salwa

Last Name

Mohammad

MiddleName

K.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Abbasia- Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Salama

MiddleName

S.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Abbasia- Cairo, Egypt

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City

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Orcid

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Volume

1

Article Issue

1

Related Issue

3481

Issue Date

2009-12-01

Receive Date

2018-10-11

Publish Date

2009-12-01

Page Start

1

Page End

19

Print ISSN

2090-0767

Online ISSN

2090-083X

Link

https://eajbsc.journals.ekb.eg/article_16301.html

Detail API

https://eajbsc.journals.ekb.eg/service?article_code=16301

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1

Type

Original Article

Type Code

673

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

Publication Link

https://eajbsc.journals.ekb.eg/

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Article

Created At

22 Jan 2023