Background: It is estimated that every year 8 million new cases of tuberculosis occur globally and that 2 to 3 million people die annually from the disease. Human genetic variation is an important determinant of the outcome of infection with Mycobacterium tuberculosis. The aim of this study is to determine genetic susceptibility to tuberculosis among patients and their control by identifying the frequency of some IFNγR1 polymorphisms in development of susceptibility to pulmonary tuberculosis in Gezira state of Central Sudan.          Methods: Polymorphism (-56 T< C) was analyzed in both 126 confirmed positive TB cases and 200 matched controls using PCR-RFLP method.          Results: The results showed that the distribution of IFNγR1-56 T < C genotype differs significantly between tuberculosis patients and controls and those carrying the mutant C allele were associated with two times reduced risk for susceptibility to Tuberculosis (P-value = 0.017, odds ratio = 1.6, 95% CI = 1.025 – 1.441), that mean the mutation IFNγR1 -56 T < C gives protection two times more than the wild type allele. However,more studies in IFNγR1 gene, and polymorphisms in IFNγ gene, needs to be performed with larger sample size