The cytochrome b gene in the salmo trutta fario has been sequenced and characterized and deposited in GeneBank, Accession Number (JN995186), the gene of cytochrome b approximately 1.2 kb. and consists of one exon from first to end of the gene, as found for  all of salmonids including, salmo salar, salmo trutta caspius, salmo trutta fario that reported in GeneBank. There were the first to end of gene repetitive regions C-G that was unique for cytochrome b gene. At this study DNA extracted from muscles of salmo trutta fario, after running PCR on the gel, the PCR products purify and sequenced. The fragments aligned with BLAST Network system, the results are shown there were high homology between solmonids, the rate of homology between salmo salar 93%, salmo trutta caspius almost 95% and salmo fario (was reported in GeneBank) 93%. However, the homology of sequences were very high, but the shape of salmo trutta fario and other salmonids different, salmo trutta fario has red to purple color dots on the skin but regards salmo salar and salmo trutta caspius have grey to black dots colors on the skin.