ABCB10 is a mitochondrial ATP-binding cassette (ABC) transporter. It is involved in multi-drug resistance and it has a potential role in the regulation of oxidative stress induced by toxic substances, such as heavy metals. Little is known about the role of ABCB10 gene in metal detoxification in fishes generally and Zebrafish particularly. Also the effect of Tubifex worms on the gene expression has not been discussed before, despite the importance of these worms in metals transfer and bioavailability. Therefore, the impact of contaminated sediment with Cu and Cu on ABCB10 gene expression was investigated in this study. In addition, the effect of Tubifex worms on the expression of Zebrafish ABCB10 gene was also studied. Zebrafish was exposed for 7 days to contaminated sediment with 315 mg/kg and 84.8 mg/kg of Cu and Cd, respectively. Relative gene expression was recorded in Zebrafish different organs (brain, gills, muscles and digestive tract), in 4 experimental groups in presence and absence of Tubifex worms. The highest level of ABCB10 expression was recorded in digestive tract samples in all tested groups followed by muscles then gills, while brain samples recorded the least induction level for ABCB10 expression. Tubifex worms showed an effective influence on Zebrafish ABCB10 expression with higher up-regulation level compared with those recorded for Cu and Cd contamination.