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16123

Transgenic Drosophila melanogaster Carrying RNAi Against gce Gene Proved the Involvement of gce in Juvenile Hormone Action

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Last updated: 22 Jan 2023

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Abstract

Juvenile
hormone (JH) is important for multiple aspects of insect development and
physiology. Although roles of JH have received considerable studies, JH
signaling pathway at the molecular level is still not well understood. Methoprene-tolerant
(Met) in Drosophila melanogaster fulfills many of the
requirements as a hormone receptor gene. A paralogous gene, germ-cell
expressed (gce), possesses homology to Met and is a candidate
as a Met partner in mediating JH action. To probe roles of this gene in
JH action, we carried out in vivo gce underexpression studies using RNAi
technique. Precocious expression of broad (br) gene was found to
occur when gce was knocked down in the young larvae. We also demonstrated
that RNA interference-driven knockdown of gce expression in transgenic
flies results in appearance of resistance to pyriproxyfen (IGRs). These results
show that gce is a vital gene and appears to promote JH action in larval
stages.

DOI

10.21608/eajbsc.2012.16123

Keywords

Drosophila melanogaster, Transgenic, Juvenile Hormone, RNAi and gce gene

Authors

First Name

Reda

Last Name

Bakr

MiddleName

F. A.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Abbassia- Cairo, Egypt

Email

redabakr55@gmail.com

City

-

Orcid

-

First Name

Jehane

Last Name

Hafez

MiddleName

A.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Abbassia- Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Thorya

Last Name

Alnagar

MiddleName

F. K.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Abbassia- Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Dalia

Last Name

Salem

MiddleName

A. M.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Abbassia- Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Ola

Last Name

Zyaan

MiddleName

H. Zyaan1

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Abbassia- Cairo, Egypt

Email

-

City

-

Orcid

-

Volume

4

Article Issue

1

Related Issue

3464

Issue Date

2012-12-01

Receive Date

2018-10-08

Publish Date

2012-12-01

Page Start

31

Page End

42

Print ISSN

2090-0767

Online ISSN

2090-083X

Article File

EAJBSC_Volume 4_Issue 1_Pages 31-42.pdf

PDF . 1.2MB

Link

https://eajbsc.journals.ekb.eg/article_16123.html

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https://eajbsc.journals.ekb.eg/service?article_code=16123

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Original Article

Type Code

673

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

Publication Link

https://eajbsc.journals.ekb.eg/

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Article

Created At

22 Jan 2023