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16099

Molecular genetic identification of two bracon species based on RAPD-PCR and 16S rRNA genes

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Last updated: 03 Jan 2025

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Abstract

Random amplified polymorphic DNA (RAPD)-PCR genomic fingerprinting and partial sequencing of the 16S rRNA gene were evaluated on two insects collected from the Egyptian field which could belong to B. hebetor and B. brevicornis to investigate their genetic relatedness and to establish the value of techniques for their identification. Nearly identical RAPD-PCR profiles and identical 467 bp fragments of the 16S rRNA genes indicated many of genetic diversity between the two insects under study. The low levels of similarity (78.21% in the partial 16S rRNA genes and 86% in RAPD-PCR) appeared between the insects B. hebetor egypt and B. brevicornis egypt. However, 16S rRNA genes and RAPD-PCR provided an effective means of differentiating between members of the taxa. Moreover, a phylogenetic tree constructed from 16S rDNA sequences showed that B. hebetor egypt clustered with the B. hebetor with a degree of similarity 92%, but B. brevicornis Egypt clustered in a separated group. However, RAPD-PCR and partial sequencing of the 16S rDNA analysis raises questions about the taxonomic positioning of the two insects isolated from the Egyptian environment.

DOI

10.21608/eajbsc.2013.16099

Keywords

RAPD-PCR and 16S rRNA genes, bracon species

Authors

First Name

Reda

Last Name

Bakr

MiddleName

F.A.

Affiliation

Department of Entomology,Faculty of Science Ain Shams University Cairo, Egypt

Email

redabakr55@gmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Gesraha

MiddleName

Ahmed

Affiliation

Pests and Plant Protection Dept., Agricultural and Biological Division, National Research Center, Dokki, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Noha

Last Name

Guneidy

MiddleName

Awny Mohamed

Affiliation

Department of Entomology,Faculty of Science Ain Shams University Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Nagy

Last Name

Farag

MiddleName

Abd Elatief

Affiliation

Pests and Plant Protection Dept., Agricultural and Biological Division, National Research Center, Dokki, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Amany

Last Name

Ebeid

MiddleName

Ramadan

Affiliation

Pests and Plant Protection Dept., Agricultural and Biological Division, National Research Center, Dokki, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Hoda

Last Name

Elbehery

MiddleName

Hassan Ali

Affiliation

Pests and Plant Protection Dept., Agricultural and Biological Division, National Research Center, Dokki, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Abou-Ellail

MiddleName

-

Affiliation

Department of Cytology and Genetics, Genetic Engineering and Biotechnology Division, National Research Centre, Cairo, Egypt

Email

-

City

-

Orcid

-

Volume

5

Article Issue

2

Related Issue

3454

Issue Date

2013-12-01

Receive Date

2018-10-07

Publish Date

2013-12-01

Page Start

99

Page End

107

Print ISSN

2090-0767

Online ISSN

2090-083X

Article File

EAJBSC_Volume 5_Issue 2_Pages 99-107.pdf

PDF . 1.0MB

Link

https://eajbsc.journals.ekb.eg/article_16099.html

Detail API

https://eajbsc.journals.ekb.eg/service?article_code=16099

Order

10

Type

Original Article

Type Code

673

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

Publication Link

https://eajbsc.journals.ekb.eg/

MainTitle

Molecular genetic identification of two bracon species based on RAPD-PCR and 16S rRNA genes

Details

Type

Article

Created At

22 Jan 2023