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16098

Modulation of autophagy enhances the apoptotic cell death in human T lymphoma cells treated with Anthocyanin

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Last updated: 03 Jan 2025

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Abstract

In the present study, the effect of modulation of autophagy induced by
anthocyanin on cell death of Human T lymphoma cells (Jurkat) was studied.
Anthocyanin was abstracted from dry petals of Hibiscus sabdariffa,
aquous solution of anthocyanin was added to cells at different concentrations
then cell viability was determined by trypan blue exclusion method. Autophagy
induced by anthocyanin was inhibited by 5 mM NH4CL which halts
lysosomal enzymes and accordingly preventing autolysosomes formation. On the
other hand, Autophagy was enhanced by glucose starvation. In both experiments
of autophagy inhibition and enhancement, cell viability was studied to
investigate the effect of autophagy modulation on cell viability. The results
of this work revealed that both inhibition and enhancement of autophagy induced
by anthocyanin lead to massive cell death. Immuno-detection of active caspase
3, one of the major hallmark of apoptotic cell death revealed remarkable
increase of active caspse3 upon autophagy inhibition or enhancement. In
conclusion, modulation of autophagy induced by anthocyanin lead to increasing
of apoptotic cell death

DOI

10.21608/eajbsc.2013.16098

Keywords

modulation of autophagy- human tlymphoma cells, Anthocyanin

Authors

First Name

Gamal

Last Name

El-Sokkary

MiddleName

H.

Affiliation

Department of Zoology, faculty of Science, Assiut University, Assiut, 71516, Egypt

Email

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City

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Orcid

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First Name

Douaa

Last Name

Sayed

MiddleName

M.

Affiliation

Department of Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, 71516, Egypt.

Email

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City

-

Orcid

-

First Name

Salwa

Last Name

Fuad

MiddleName

A.

Affiliation

Department of Zoology, faculty of Science, Assiut University, Assiut, 71516, Egypt

Email

-

City

-

Orcid

-

First Name

Abo Bakr

Last Name

Abdel Shakor

MiddleName

M.

Affiliation

Department of Zoology, faculty of Science, Assiut University, Assiut, 71516, Egypt

Email

eltayeb702002@yahoo.com

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Orcid

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Volume

5

Article Issue

2

Related Issue

3454

Issue Date

2013-12-01

Receive Date

2018-10-07

Publish Date

2013-12-01

Page Start

87

Page End

97

Print ISSN

2090-0767

Online ISSN

2090-083X

Article File

EAJBSC_Volume 5_Issue 2_Pages 87-97.pdf

PDF . 438.1kB

Link

https://eajbsc.journals.ekb.eg/article_16098.html

Detail API

https://eajbsc.journals.ekb.eg/service?article_code=16098

Order

9

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Original Article

Type Code

673

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

Publication Link

https://eajbsc.journals.ekb.eg/

MainTitle

Modulation of autophagy enhances the apoptotic cell death in human T lymphoma cells treated with Anthocyanin

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Article

Created At

22 Jan 2023