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16045

Molecular characterization of Paenibacillus larvae larvae, for early diagnosis of american foulbrood of honeybees in Egypt

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Last updated: 03 Jan 2025

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Abstract

A reliable procedure for early detection of Paenibacillus larvae subsp. larvae (P. l. larvae), the causal agent of American Foulbrood disease (AFB) of honeybees (Apis mellifera L.) based on the polymerase chain reaction (PCR) and subspecies – specific KAT primers. A PCR amplicon of the expected size 550 bp only found in P. l. larvae strains was used for positive AFB. This PCR assay provides a specific detection for P. l. larvae from week 1 post infection even if there is no clinical symptoms appeared in a colony. The technique can be directly used to detect presence or absence of P. l. larvae spores in honeybee samples and contaminated honeys.

DOI

10.21608/eajbsc.2014.16045

Keywords

Molecular-, Paenibacillus larvae, Honeybees, American foulbrood, Egypt

Authors

First Name

Shireen

Last Name

Ma'moun

MiddleName

A. M.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Imam

MiddleName

I.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Emad

Last Name

Nafea

MiddleName

A.

Affiliation

Honeybees' Research Center, Plant Protection Institute, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Rabia

Last Name

Anan

MiddleName

A.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

Salama

MiddleName

S.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Akila

Last Name

El Shafai

MiddleName

M.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

Kaschef

MiddleName

H.

Affiliation

Department of Entomology, Faculty of Science, Ain Shams University, Cairo, Egypt

Email

-

City

-

Orcid

-

Volume

6

Article Issue

1

Related Issue

3452

Issue Date

2014-06-01

Receive Date

2018-10-06

Publish Date

2014-06-01

Page Start

35

Page End

45

Print ISSN

2090-0767

Online ISSN

2090-083X

Article File

EAJBSC_Volume 6_Issue 1_Pages 35-45.pdf

PDF . 412.6kB

Link

https://eajbsc.journals.ekb.eg/article_16045.html

Detail API

https://eajbsc.journals.ekb.eg/service?article_code=16045

Order

4

Type

Original Article

Type Code

673

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology

Publication Link

https://eajbsc.journals.ekb.eg/

MainTitle

Molecular characterization of Paenibacillus larvae larvae, for early diagnosis of american foulbrood of honeybees in Egypt

Details

Type

Article

Created At

22 Jan 2023