Background: Type 2 diabetes is the commonest form of diabetes and associated with multiple metabolic derangements that result in the excessive production of reactive oxygen species (ROS) and oxidative stress, the aim of present study was to Determined of Lipid Per oxidation , Lipid profile and Vitamins A, E In Sudanese with Type ll Diabetes Mellitus. Methodology:100 healthy subject were control group with mean FBS= 5.61m. mol/L,. The age ranged from (25-78) years old. The mean age average was50.2(years). Results: shows significant raised of the means of the plasma levels of malondialehyde, fasting plasma glucose, cholesterol, LDL, triglyceride, and HbA_1c of the test group when compared with healthy control group subjects, whereas the means of the plasma levels of antioxidant vitamins (A, E),and HDL showed significant reduction when compared with that of control groupsignificant difference when compared with healthy subjects. Conclusion: The present study shows that significant lipoprotein abnormalities in type 2 diabetic patients when compared with healthy subjects. The increased level of serum MDA and lower level of serum vitamin E clearly shows that diabetic patients was exposed to an increased oxidative stress via lipid peroxidation.