The fish were divided into four groups, control group and three groups injected intramuscularly with follicular stimulating hormone and leutinizing hormone (FSH and LH) 10, 20 and 40 μg kg− 1 body weight dissolved in 0.6% NaCl under a natural temperature regime. The fish were held in large tanks contained water from their farm. The fish were fed with 30 % protein diet during experiment. Females and males Oreochromis niloticus 150 - 160 g body weight from Abbasa farm were injected with a doses of follicular stimulating hormone and leutinizing hormone (10, 20 and 40 μg kg− 1 body weight) three times a week,  The result showed multiple ovulations and spawns within a period of approximately 30 days. Plasma levels of 17β-estradiol (E2), follicular stimulating hormone (FSH) and leutinizing hormone (LH) were elevated at 30 days post-treatment, preceding the spawns with the highest fecundity, concomitant with a decrease in the levels of plasma testosterone (T). However, follicular stimulating hormone and leutinizing hormone (10, 20 and 40 μg kg− 1 body weight) treatment in males increased plasma levels of testesterone and 11-ketotestosterone (11-KT) during injection. These results show that administration of follicular stimulating hormone and leutinizing hormone (10, 20 and 40 μg kg− 1 body weight) induces multiple spawns in Oreochromis niloticus. where, approximately 100% increase in red blood cells (RBCs), white blood cells (WBCs), hematocrit value (Hc) and hemoglobin content (Hb) respectively. Also, there are increased in serum glucose level, total protein, aspartate and alanine amino transferase activities (AST and ALT) and increased serum creatinine and uric acid concentration after administration of follicular stimulating hormone and leutinizing hormone (10, 20 and 40 μg kg− 1 body weight) respectively. The results indicate that the fish that administration of follicular stimulating hormone and leutinizing hormone (10, 20 and 40 μg kg− 1 body weight)  increase blood constituents, serum glucose level, serum total protein and serum AST, ALT activities, creatinine and uric acid level. These data suggest that fish respond to administration of follicular stimulating hormone and leutinizing hormone (10, 20 and 40 μg kg− 1 body weight) by increasing their WBCs, enzymes of liver functions and kidney functions.