Infection with hepatitis C virus (HCV) is a common global cause of chronic liver disease, which is also true in Saudi Arabia. HCV prevalence in Saudi Arabia varies in different provinces being highest in the Western and Southern provinces. Most of the studies among blood donors documented a decrease in HCV prevalence, probably due to increase awareness and improved socioeconomic status. The present study was designed to investigate the prevalence of hepatitis C infection among general population of Hail region, Saudi Arabia and to determine titer of anti-HCV in patient serum. A total of 100 participants in he study survey with average age of 18-63 years, were preformatted a questionnaire including the demographic data, socio-economic status and medical history. Three mL of venous blood were collected from 37 participants, in a container with strict aseptic precautions. Collected serum samples were used for serological evaluation of anti-HCV infection, by using ELISA. Among the participants in the study survey ,HCV positivity percentage was found to be (1%) (One /100) in our study. Thirty seven serum samples were examined for anti-HCV, thirty of them were positive with titer ranged from (10.512 to 1859.395 IU/ml), while seven samples were negative, In conclusion, the obtained data revealed, low prevalence of HCV in Hail region, Saudi Arabia.