The basidiomycete fungus Ganoderma Karst., a polyporoid genus within the family Ganodermataceae of the order Aphyllophorales, is worldwide in distribution. The accurate identification of the Ganoderma is still controversial particularly for the tropical species due to high variability in the basidiocarp morphology, and complicated speciation which leads to misidentification by traditional taxonomic methods. Specimen of Ganoderma basidiocarps were collected from different hosts (Navel orange, Oil palm, Fan palm, Casuarina and Morus) in Giza and Qalyubia governorates, Egypt and were identified to species level according to its morphological characters  as well as PCR and nucleotide sequence analysis of the ribosomal 5.8S r-DNA gene and the flanking internal transcribed spacers (ITS) utilizing specific primers with ITS 1 region as a target. Isolates of Ganoderma resinaceum were described for the first time in Egypt, where, morphological and cultural observations and phylogenetic analysis of ITS1 sequences revealed that all isolates collected from infected trees belong to a single species Ganoderma resinaceum.