The aim of this study was focused on the possible protective effect of L-carnitine against gamma radiation induced cardiotoxicity in male albino rats. Forty albino rats were divided into four equal groups as follows: Control group without radiation (placebo), L-carnitine treated group (rats were given orally L-carnitine at a dose of 300 mg/kg/day), irradiated group (animals subjected to whole body gamma irradiation at a dose level of 6Gy)and irradiated group pretreated with L-carnitine (animals were treated orally with L-carnitine at a dose of 300 mg/kg/day before irradiation then exposed to whole body gamma irradiation at a dose of 6Gy). Cardiotoxicity was assessed by measuring the serum levels of CK, CK-MB, LDH, AST, cTnI, TAC, MDA and lipid profile. The obtained results revealed that the administration of L-carnitine to irradiated rats significantly ameliorated the changes occurred in the investigated biochemical parameters. In conclusion, L-carnitine acts as a potent scavenger of free radicals to prevent or ameliorate the toxic effects of gamma irradiation. Also, L-carnitine might provide substantial protection against radiation-induced cardiotoxicity.