It has been concluded from several studies that Gum Arabic (GA) offers a protective effect as an anti-inflammatory and antioxidant agent against nephrotoxicity induced by some agents such as adenine and gentamicin. . In this study, the protective and/or treatment effect of GA against Cisplatin-induced acute nephrotoxicity in experimental rats were investigated through several biochemical, histological and immunohistochemical assessment of TGF- beta. Thirty male Wister albino rats were divided randomly into five groups, six rats in each. Group I (negative control), group II (GA) received 6% GA in drinking water, group III (Cisplatin) injected by CP (4.5 mg/kg b.w., i.p.) for two consecutive days, group IV (preventive) pretreated with GA 6% in drinking water daily for 4 weeks before Cisplatin injection and group V (treatment) co-treated by GA with Cisplatin injection (doses as mentioned before). Group III (Cisplatin) showed nephrotoxicity that was manifested by a significant increase in levels of serum Creatinine, urea, and potassium with elevation in kidney MDA level and a decrease in kidney GSH level. In addition, histopathological examination showed severe degeneration and necrosis in kidney tubules. These effects were significantly mitigated by GA administration in both groups IV and V, a result that proves the reno-protective effect of GA.