Background: It is well understood that a major contributing factor to male infertility is sperm DNA breakdown. Many studies have been carried out to investigate how sperm DNA damage affects the patient reproduction ability. This suggests different outcomes, and the subject remains contentious. We investigated differences in sperm parameters and embryonic developmental steps in our study, and the observations compare the results of ICSI using sperm extracted from testicular on patients with high sperm injury to the results of patients with sperm DNA fragmentation on ejaculate samples. Materials and Methods: From March 2017 to December 2019, 418 couples were enrolled in our study at the Algazeera Centre for IVF Giza, Egypt. The parameters of the sperm were evaluated. The value sperm DNA fragmentation index (DFI) was calculated desponded on the sperm chromatin dispersion (SCD) diagnostic test, which was applied to teams in the regulation (sperm DNA fragmentation index < 30%) or abnormal (sperm DNA fragmentation index > 30%) groups. Results: decrease in fertilization and blastulation rate in a group of patients suffering high sperm damage on ejaculate samples and our results show no difference between normal ejaculate samples and testicular sperm extrication ( SDF) groups. Conclusion: no impact was detected on ICSI outcomes (pregnancy rates) between the three groups but a negative effect for sperm DNA fragmentation on fertilization and blastulation rate and blastulation rate depended on the age of women and the number of embryos transferred.