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210632

Bioactive Compounds of Ziziphus spina-christi Seeds Extract and Cellulase Enzyme Attenuates the Growth of Acanthamoeba polyphaga Isolated From Contact Lenses

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Last updated: 03 Jan 2025

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Abstract

Background: Free-living Acanthamoeba spp. can cause sight-threatening amoebic keratitis and fatal granulomatous amoebic encephalitis. The difficulties in protecting against Acanthamoeba spp. frequently begin with a lack of diagnosis and continue with a lack of treatment. The current study aimed to evaluate the efficacy of ethanolic extract from Ziziphus spina christi (ZSC) seeds and cellulase enzyme as potential treatments against Acanthamoeba polyphaga compared to chlorhexidine (CHX) treatment. Methodology: Acanthamoeba polyphaga were isolated from contact lenses and contact lens solutions and were observed daily for72–96 h and 3 weeks for trophozoites and cysts, respectively. Five groups, including ZSC, cellulose enzyme, the combination of ZSC and enzyme, CHX group, and control group < /em> were designed. Results: GC-MS analysis of the extract revealed ~ 85 bioactive compounds (primarily fatty acids and fatty acid derivatives). The antioxidant capacity of the extract at 800, 500, and 200 mg/ml was 1.972, 1.542, and 0.958 mg of ascorbic acid/g dry weight, respectively. Light and scanning electron microscopy observations revealed degeneration, decreasing in size, and distortion of the trophozoites and cysts.The viability of trophozoites and cysts was significantly reduced by different concentrations of the extract either alone or in combination with cellulose enzyme compared to 0.02% CHX. Conclusion: These results indicate that ethanolic extract from ZSC seeds (at the tested concentrations) and cellulase enzyme have anti-Acanthamoeba potential at various incubation periods.

DOI

10.21608/eajbsz.2021.210632

Keywords

Acanthamoeba, ziziphus, GC-MS analysis, DISTORTION, viability

Authors

First Name

Sara

Last Name

Abdel-Hakeem

MiddleName

salah

Affiliation

Parasitology Laboratory, Zoology Department, Faculty of Science, Assiut University, Assiut (71526), Egypt

Email

sara_assiut86@aun.edu.eg

City

-

Orcid

-

First Name

Hossam El- din

Last Name

Omar

MiddleName

Mohamed

Affiliation

Physiology laboratory, Zoology Department, Faculty of Science, Assiut University, Assiut (71526), Egypt

Email

hossameldin.mo@aun.edu.eg

City

-

Orcid

-

First Name

Gamal

Last Name

Abed

MiddleName

Hassan

Affiliation

Parasitology laboratory, Zoology Department, Faculty of Science, Assiut University, Assiut (71526), Egypt

Email

abed@aun.edu.eg

City

-

Orcid

-

First Name

Faten

Last Name

Hassan

MiddleName

Abdo Mohammed

Affiliation

Parasitology laboratory, Microbiology Department, Faculty of Science, Taiz University, Taiz, Yemen

Email

fatenhassan28@yahoo.com

City

-

Orcid

-

First Name

Osama

Last Name

Al-Bedak

MiddleName

-

Affiliation

Assiut University Mubasher Mycological Centre, Assiut University, Assiut, Egypt

Email

osamaalbedak@gmail.com

City

-

Orcid

-

First Name

Mohammed

Last Name

Tolba

MiddleName

Essa Marghany

Affiliation

Parasitology Department, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

essa3eg@aun.edu.eg

City

-

Orcid

-

Volume

13

Article Issue

2

Related Issue

26137

Issue Date

2021-12-01

Receive Date

2021-11-12

Publish Date

2021-12-01

Page Start

307

Page End

321

Print ISSN

2090-0759

Online ISSN

2090-0821

Article File

EAJBSZ_Volume 13_Issue 2_Pages 307-321.pdf

PDF . 1.5MB

Link

https://eajbsz.journals.ekb.eg/article_210632.html

Detail API

https://eajbsz.journals.ekb.eg/service?article_code=210632

Order

25

Type

Original Article

Type Code

672

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, B. Zoology

Publication Link

https://eajbsz.journals.ekb.eg/

MainTitle

Bioactive Compounds of Ziziphus spina-christi Seeds Extract and Cellulase Enzyme Attenuates the Growth of Acanthamoeba polyphaga Isolated From Contact Lenses

Details

Type

Article

Created At

22 Jan 2023