The objective of this study was to analyze the potential toxicity of the Hydroxyapatite. 60 male albino rats were treated with Hydroxyapatite for 30 days. Toxicity was monitored by quantitative analysis of the serum testosterone hormone level; follicle-stimulating hormone (FSH) level in serum, luteinizing hormone (LH) level in serum, prolactin hormone PRL) level in serum, AST activity and Alkaline phosphatase activity was measured and tests were collected for histopathological study. The results refer to a significant decrease in testosterone; FSH, LH and PRL while a significant increase in AST and ALP). Histopathology revealed moderate disturbance with an irregular cycle of spermatogenesis in seminiferous tubules, a marked decrease in the number of spermatogenic cells in the seminiferous tubules, a marked decrease in the number of sperms and Leydig cells. The administration of the Chitosan had beneficial and decrease side effects against the deleterious changes of Hydroxyapatite. In conclusion, results suggest a potential contribution of Hydroxyapatite to the etiology of somebody's diseases while Chitosan has beneficial effects as it tends to dampen Hydroxyapatite toxicity in rats.