The present work aims to investigate the effect of Chitosan (as a natural adsorbent) for removing five heavy metals from synthetic polluted water after different contacting periods to be suitable for aquaculture and other purposes. Three different concentrations: 5, 7.5 and 10 g / l of chitosan were tested for their removal efficiency for 1 ppm of Fe, Zn, Cu, Cd and Pb at different contact periods. The effect of different contacting periods (30 Min, 1, 3, 6, 12, 18 and 24 hours) was tested with the three investigated concentrations of chitosan on the removal efficiency of the five metals. It's revealed that the removal efficiency increases with the increase in the contracting period, where the lowest values of removal efficiency for Fe, Zn, Cu, Cd and Pb were 27.7, 48.4, 68.4, 22.2 and 88.3 %, respectively after 30 Minutes, while the highest ones were 95.2, 91.9, 89.1, 94.1 and 99.9 %, respectively after 24 hours. The equilibrium adsorption behaviour data were fitted well to Langmuir and Freundlich adsorption isotherm models. R² for Langmuir values for removing the investigated metals ranged between (0.947 to 1), while for Freundlich R² values ranged between (0.931 to 0.992). Isothermal studies proved that chitosan was highly potential for removing all metals from polluted water.