The present survey of mite species inhabiting organic manure in different animal-sheds (i.e., buffalo, cow, sheep, goats), in both farms, the experimental farm of the Faculty of Agriculture, Assiut University and the production farm of El-Fateh district, Data showed that there were 28 mite species pertaining to 20 genera of 12 families in three mite orders (Mesostigmata, Astigmata, Trombidiformes). Mesostigmata represented by 19 species (8 families); Astigmata (Acarididia), represented by 8 species (2 families) and Trombidiformes represented by two species belonging to two families. Both mite species of the families Macrochelidae Vithzthum, 1930, and Parasitidae Oudemans, 1901 were recorded in high densities. While scarce numbers of Tarsonemidae Canestrini & Fanzago, 1876 were observed. The annotated checklist reported 150 mite species belonging to 67 genera of 26 families.