The experiment was conducted at the Fish Hatchery - Department of Fisheries and Wildlife Science, College of Animal Production Science and Technology, Sudan University of Science and Technology during the period 15/07-02/09/2018 to determine the effect of replacing fishmeal with baobab seed meal in the formulation of diets for Nile Tilapia (O. niloticus) fish and determining effect on the growth rate. The experiment included 12 plastic aquariums, Fish were distributed randomly in aquariums and placed in each one 10 fish. Acclimatized to the hatchery conditions for 3 days, before the beginning of the experiment. The experiment included 4 treatments with 3 replicated aquariums for each. Feeds T0, T1, T2 and T3 (The diets replacing 0, 25, 50 and 75% of fish meal protein content by baobab seed meal. The results indicated that final body weight (BW), weight gain (WG) and specific growth rate (SGR) of O. niloticus increased with increasing level of fish baobab seed meal in diets. WG was found 20.10, 28.23 and 37.90 for T1, T2 and T3 respectively. The data were analysed by one-way analysis of variance (ANOVA, F test) and LSD for significantly different means at a significance level of 0.05 using SPSS version 16.