Objective: A questionnaire–based prevalence study was carried out on a random sample from various girls' science college, KingKhalidUniversity, to find out the distribution pattern of ABO and Rh blood groups among Saudi females, which would not only help in blood transfusion services but also eliminate the risk of erythroblastosis fetalis in the neonates. Method:  The procedure for this study was done by giving the questionnaire to 3287 female students. Each participant filled out the questionnaire and gave it back after answering all the questions. The participants were volunteered to fill out the questionnaire. Results: Of the 3287 female students enrolled in this study, 965 students know their blood groups. Amongst ABO system, blood group O was the most common (57.31%) followed by A (32.74%), B (9.22%), and AB (0.73%), respectively. Regarding rhesus system, Rhesus positive was more common (90.3%) than Rhesus negative (9.7%). Conclusion: The current study documents ABO and Rhesus blood group distribution pattern amongst King Khalid University Girls Students. Findings ensured that there is need for educational and community support program for effective management of medical emergencies.