Water Toxicity is a worldwide problem, heavy metals belonging to the most important pollutants. The progress of industries has led to increased emission of pollutants into ecosystems. The fish were divided into four groups, control group and three groups exposed to 5, 10 and 15 mg/l of lead acetate, 10, 20 and 30 mg L-1 of ferric chloride and 10, 20 and 300 mg L-1 of zinc sulfate respectively, the experimental fish were exposed to treatment for periods of 15 days, 30 days and 45 days. The Nile tilapia was exposed to water pollution with lead, iron and zinc. The Nile tilapia 80 - 100 gm body weight was obtained from Abbassa farm. The fish were reared in large tanks contained water from their farm. The fish were acclimatized and fed on 30 % protein diet. Result of the present study revealed that lead concentrations in the tissues of the fish were elevated and highest contamination were increased in the gill and liver than that in the muscles. Also, there was vary significant increase in the concentrations of lead in the gill, liver and muscles tissues of Oreochromis niloticus exposed to different concentrations of lead acetate than that control fish tissues. Iron concentrations in the tissues of the fish were elevated in the gill and liver than that in the muscles. Also, there was vary significant increase in the concentrations of iron in the gill, liver and muscle tissues of O. niloticus exposed to different concentrations of ferric chloride at different period than that control fish tissues. Zinc concentrations in the gill and liver were higher than that in the muscles. Also, there was significant increase in the concentrations of zinc in the gill, liver and muscle tissues of O. niloticus exposed to different concentrations of zinc sulfate at different period than that control fish tissues. Results generally showed that metal concentrations were always lowest in the muscles and highest in the gill and liver.