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13462

New Approaches For Extracting And Mass Rearing of Entomopathogenic Nematodes In Vivo

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Last updated: 22 Jan 2023

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Abstract

The dual-purpose of spongy traps for extracting and storage of EPNs was validated in this investigation. The spongy traps proved to be the most efficient method for recovering IJs from the insect cadavers (Galleria mellonella) comparing with the traditional white traps. Also, the spongy trap kept the viability of IJs for prolonged time (12 weeks). Based on these results, duplications of insect cadavers in spongy traps will be rather encouraging for nematode mass production. A linked study was continued for detection an artificial diet for G. mellonella larvae. This step is necessary towards the mass production of EPNs in vivo. The recommended artificial media divided into two parts: the first part is supplemented with old beewax used for feeding 1st and 2nd instars of insect larvae. The second part is supplemented with paraffin oil used for feeding the other instars of insect larvae. The present results demonstrated that, the insect larvae could live and develop on this artificial diet. Moreover, both nematodes of Heterorhabditis bacteriophora (Hb) and Steinernema carocapcae (Sc) have proven to propagate perfectly in insects fed on the artificial media. Full death of all treated insects by Sc or Hb occurred (mortality=100%). The pathogenicity and reproduction of Hb nematodes produced from insect larvae fed on artificial diet gave initial population (Pi), final population (Pf), Rate of reproduction (Rr) and Efficiency of conversion (Ec) values superior than those fed on beewax checks at 100 IJs/insect larva. Similary, the pathogenicity and reproduction of Sc nematodes produced from insect larval fed on artificial diet were equivalent to or superior than those produced from insects fed on beewax in all inoculum levels. These new methods require minimal expertise and capital investments for extracting, storage and mass production of the beneficial Entomopathogenic nematodes (EPNs).

DOI

10.21608/eajbsz.2016.13462

Keywords

Extracting, storage EPNs, Heterorhabditis bacteriophora, Steinernema carocapcae, Galleria mellonella, artificial media

Authors

First Name

A.

Last Name

Kassab

MiddleName

S.

Affiliation

Department of Plant Protection, Faculty of Agriculture, Ain Shams University

Email

entsarhelmy784@gmail.com

City

-

Orcid

-

First Name

Entsar

Last Name

Taha

MiddleName

H.

Affiliation

Department of Plant Protection, Faculty of Agriculture, Ain Shams University

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City

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Orcid

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Volume

8

Article Issue

1

Related Issue

2592

Issue Date

2016-08-01

Receive Date

2018-09-12

Publish Date

2016-08-01

Page Start

39

Page End

47

Print ISSN

2090-0759

Online ISSN

2090-0821

Link

https://eajbsz.journals.ekb.eg/article_13462.html

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https://eajbsz.journals.ekb.eg/service?article_code=13462

Order

4

Type

Original Article

Type Code

672

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, B. Zoology

Publication Link

https://eajbsz.journals.ekb.eg/

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Article

Created At

22 Jan 2023