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26985

Evaluation of The Cytotoxicity, Cell Cycle Perturbations and Apoptotic Induction in Human Normal and Cancer Liver Cell Lines Exposed to Potassium Nitrate and Sodium Benzoate

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Last updated: 03 Jan 2025

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Abstract

  This proposal investigates in vitro cytotoxic and apoptotic effects of two food preservatives commonly consumed in daily meals; potassium nitrate and sodium benzoate. The effect of these two preservatives on cell viability was tested on two different cell lines; normal liver cell line THLE2 and human hepatocellular carcinoma cancer cell line HepG2 using MTT assay. Although the effect was more significantly realized in HepG2 cell line, the viability of both cell lines was decreased by both of the tested compounds. Flow cytometric analysis of the HepG2 treated cells has revealed an increase in G2/M phase cell cycle arrest. Measurement of expression levels of three central genes; p53, bcl-2 and bax that play key roles in cell cycle and apoptosis were carried out in HepG2 using real time-PCR and western blot. In conclusion, both of the tested compounds have decreased the cell line viability and induced both cell cycle arrest and apopototic events indicating their high potential of being cytotoxic and genotoxic materials.   

DOI

10.21608/eajbsz.2018.26985

Keywords

cytotoxic, Genotoxic, cell cycle arrest, Real-Time PCR, western blot, food additives

Authors

First Name

Amal

Last Name

Aledwany

MiddleName

Z.

Affiliation

Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt

Email

-

City

-

Orcid

-

First Name

Wesam

Last Name

Basal

MiddleName

T.

Affiliation

Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt.

Email

-

City

-

Orcid

-

First Name

Neima

Last Name

Al-Senosy

MiddleName

K.

Affiliation

Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.

Email

neimakotb@yahoo.com

City

-

Orcid

-

First Name

Aliaa

Last Name

Issa

MiddleName

M.

Affiliation

Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt.

Email

-

City

-

Orcid

-

Volume

10

Article Issue

1

Related Issue

2587

Issue Date

2018-06-01

Receive Date

2018-04-30

Publish Date

2018-06-01

Page Start

105

Page End

118

Print ISSN

2090-0759

Online ISSN

2090-0821

Article File

EAJBSZ_Volume 10_Issue 1_Pages 105-118.pdf

PDF . 839.3kB

Link

https://eajbsz.journals.ekb.eg/article_26985.html

Detail API

https://eajbsz.journals.ekb.eg/service?article_code=26985

Order

10

Type

Original Article

Type Code

672

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, B. Zoology

Publication Link

https://eajbsz.journals.ekb.eg/

MainTitle

Evaluation of The Cytotoxicity, Cell Cycle Perturbations and Apoptotic Induction in Human Normal and Cancer Liver Cell Lines Exposed to Potassium Nitrate and Sodium Benzoate

Details

Type

Article

Created At

22 Jan 2023