This study aimed to determine the effect of different salinity and anti-transpiration levels on the growth and biochemical composition of Panicum maximum plants (Guinea grass) during spring and summer in 2020. Two different anti-transpiration treatments [molasses (sugarcane) (5 mL/L), kaolin (50 g/L), with control] and three salinity levels (S1 well water as the control and salinity S2 2000 ppm; S3 4000 ppm, S4 6000 ppm] were used in 12 treatments in 72 pots [3 anti-transpiration treatments × 4 salinity treatments × 6 replicates]; a randomized complete design was used. Results revealed that the plants achieved the highest plant height (123.77 cm) and dry weight (521.87 g/m²) with kaolin and no salt addition treatment during summer. Higher percentage of proteins and carbohydrates were found in spring than in summer; but there was no significant difference among the salinity levels. A high percentage of ash and fiber contents was also observed during summer, with no significant differences between the anti-transpiration treatments. It could be concluded that P. maximum, as one of the most important fodder crops, could be cultivated in marginal lands, especially during summer season.