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134861

Effect of Green Laser Irradiation on Epididymal Camel Spermatozoa Quality Stored at 5°C

Article

Last updated: 22 Jan 2023

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Abstract

Total number of forty testes from twenty Sudani camels (Camelus dromedarius) were used in the present study (>5-10 years old and 500-600 kg body weight). The experimental work was executed to define the effect of green laser irradiation with short-wavelength 532 nm and continuous wave from a diode laser light with a total output power of 3 mW on epididymal camel spermatozoa quality at different exposure times of 0 (control, non-irradiated), 2, 4, 6, 8, and 10 min. Following irradiation, the percentages of motile spermatozoa, storagability, viability, and acrosomal damage were assessed of the epididymal camel spermatozoa stored at 5°C for 4 days. Epididymal spermatozoa was diluted with lactose-yolk-citrate (LYC) extender. The obtained results showed that the highest (P<0.05) value of the percentages of motile and storagability of spermatozoa was recorded with spermatozoa exposed to 6 min of laser irradiation and the lowest (P<0.05) value was recorded with the control group. Otherwise, the highest (P<0.05) value of the percentages of dead and acrosomal damage of spermatozoa was recorded with spermatozoa exposed to 10 min and the lowest (P<0.05) value was recorded with 2 min. The advancement of storage time at 5°C decreased (P<0.05) the percentages of motile and storagability of spermatozoa, while increased (P<0.05) the percentages of dead and acrosomal damage of spermatozoa during storage at 5°C for 4 days. Consequently, enhancing the artificial insemination program can be achieved using the laser irradiation which is considered a cost-effective technique for improving semen quality. The profitable effects of laser irradiation on epididymal camel spermatozoa quality raised the motile spermatozoa, storagability, livability, acrosomal integrity which consider an indicator to improve mitochondrial function which extends the survival of spermatozoa

DOI

10.21608/ajs.2021.48967.1290

Keywords

camels, epididymal spermatozoa, green laser irradiation, viability, storagability

Authors

First Name

Ahmed

Last Name

Seioudy

MiddleName

-

Affiliation

Camel Research Department, Animal Production Research Institute, Agricultural Research Center, Giza, Egypt.

Email

ahmedseioudy@gmail.com

City

Cairo

Orcid

0000-0001-6627-0781

First Name

Esmat

Last Name

Abdalla

MiddleName

Bakry

Affiliation

Animal Production Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.

Email

esmat54@hotmail.com

City

Cairo

Orcid

-

First Name

Alaa

Last Name

Zeidan

MiddleName

El-Sayed

Affiliation

Camel Research Department, Animal Production Research Institute, Agricultural Research Center, Giza, Egypt.

Email

aebzeidan1961@hotmail.com

City

Cairo

Orcid

-

First Name

Farouk

Last Name

Khalil

MiddleName

Abdalla

Affiliation

Animal Production Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.

Email

faroukkhalil22@yahoo.com

City

Cairo

Orcid

-

First Name

Zienab

Last Name

Abdel-Salam

MiddleName

-

Affiliation

National Institute of Laser Enhanced Sciences, Cairo University, Giza, Egypt.

Email

zienabzz@niles.edu.eg

City

Cairo

Orcid

-

First Name

Magdy

Last Name

Badr

MiddleName

Ramadan

Affiliation

Animal Reproduction Research Institute, Agricultural Research Center, Giza, Egypt.

Email

magdybadr69@yahoo.com

City

Cairo

Orcid

-

First Name

Maiada

Last Name

Allam

MiddleName

Wagdy

Affiliation

Automated El-Bassatein Abattoir, Directorate of Veterinary Medicine, Cairo, Egypt.

Email

maiada_wagdy@yahoo.com

City

Cairo

Orcid

-

Volume

29

Article Issue

1

Related Issue

25482

Issue Date

2021-04-01

Receive Date

2020-11-21

Publish Date

2021-04-01

Page Start

307

Page End

314

Print ISSN

1110-2675

Online ISSN

2636-3585

Link

https://ajs.journals.ekb.eg/article_134861.html

Detail API

https://ajs.journals.ekb.eg/service?article_code=134861

Order

138

Type

Original Article

Type Code

668

Publication Type

Journal

Publication Title

Arab Universities Journal of Agricultural Sciences

Publication Link

https://ajs.journals.ekb.eg/

MainTitle

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Details

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Article

Created At

22 Jan 2023