The impact of nano silver & nano chitosan particles on sterilization, nano Fe and Zn on callus formation of immature inflorescence of date palm cv. Barhee during the establishment stage was investigated with immersion and adding to MS culture medium.Thelowest total contamination percentage and the highest survival percentage were achieved with nano silver particles at200 mg/l, and nano chitosan at 150, and 200 mg/l. The lowest contamination %recorded in medium culture containing silver nanoparticles at 4mg/l with NAA at 100mg/l and chitosan nanoparticles at 4 mg/l with 2,4-D at 100 mg/l. The optimum callus formation percentage and callus size were obtained on MS medium supplemented with picloram at 8 mg/l. The highest callus weight and size were showed with NAA at 10 mg/l, 2ip at 6mg/l & Kin at 6 mg/l during callus proliferation. In multiplication stage, the highest number of shoot / culture were occurred on MS medium culture supplemented with Fe nano particles at 20.8 mg/l , MS medium culture supplemented by Fe nano particles at 27.8 mg/l and Zn nano particles at 4.3 mg/l in the first subculture without any significant differences among them. The highest average shoot length (cm) was obtained with MS medium containing Fe nano particles at 27.8 mg/l, MS medium supplemented by Fe nano particles at 20.8 mg/l and Zn nano particles at 4.3 mg/l in the first subculture without any significantly differences among them. Interaction between cytokinins and auxin concentration, indicated, the highest number of shoots / culture were achieved with NAA at 2.0 mg/l, 2ip at 4 mg/l, kin at 4mg/l during the 1 st, 2 nd & 3 rd subcultures, respectively. The highest rooting percentage and number of roots/ microshoots were obtained with MS containing NAA at 0.5 mg/l. The highest survival percentages in acclimatization stage were occurred with medium mixtures of sand: peat: vermiculite: perlite at (1:2: 1:1) and (2: 1: 1:1), respectively.