A total number of 500 Japanese quail fertile eggs were used to study the effect of in ovo (I O) administration of Nano-Selenium (Nano - Se) and Glutamine (Gln) on hatchability, post hatch performance carcass and some blood biochemical traits. Eggs were divided into five treatments groups and injected just before incubation. The 1 st group was not injected (Negative Control; T 1), the 2 nd one was injected with Bovine Serum Albumin (B S A) (Positive control; T2), the 3 rd group was injected with Nano-Se at 2.5 p p b / egg (T 3), while, the 4 th (T 4) and 5 th (T 5) groups were injected with glutamine at 20 and 10 p p m / egg, respectively. The hatched chicks were distributed according to their treatments and reared up to the 6 th week of age. Parameters such as the hatchability, hatchlings weight, productive performance, carcass traits and some blood biochemical indices were estimated. The I O - Gln at 20 ppm (T 4) significantly improved hatchability, increased the productive performance of post-hatch quails (carcass weight, gizzard and breast muscles percentages) and enhanced the feed conversion ratio (F C R) compared to other groups. Furthermore, I O with Nano - Se (T 3) significantly increased plasma albumin and albumin / globulin (A / G) ratio, and decreased cholesterol and triglycerides levels, while high density lipoprotein (H D L) were significantly increased and low density lipoprotein (L D L) were significantly decreased by I O - Gln (T 4 and T 5). It could be concluded that using the in ovo administration of Nano - Se and Gln concentration for Japanese quail breeder eggs, had no negative effect on hatchability and could improve post-hatched performance carcass traits, particularly using Gln at a dosage of 20 p p m / egg.