Two famous Citrus rootstocks sour orange (C. aurantium.) and volkamer lemon (C. volkamariana.) were tested as interstocks at the stage of transplant production to investigate their mutual effects on the scion growth characteristics, mineral content, carbohydrates, total indols and phenols from side; and rootstock growth traits. nitrogen content, carbohydrates, indols and phenols from the other side. Also the results indicated that Navel orange budded on C. volkamariana grafted on C. volkamariana achieved significantly the highest scion height, root length, leaf number. The highest content of N, P, K, Mg, Fe, Zn, Mn and total phenols in scion stem. Moreover, the mentioned treatment gained the highest significant carbohydrates in roots.