This investigation was carried out through two successive seasons (2014 and 2015) to investigate the effects of nano-carbon and nitrogen fertilization levels on growth, leaf mineral content, yield and fruit quality of 3 years old flame seedless grapevines, cultivated in a private orchard with loamy soil texture, at Gharbiya Governorate. Ten treatments were applied as a randomized complete block design with three replications. The result indicated that 80%N from recommended requirment+0.6% carbon nano tubes (CNTs) increased significantly leaf area, fresh and dry weight, total carbohydrate% and concentration of N, P, K, Mg, and Fe in leaves, weight of 100 berries,  and juice weight of 100 berries compared with control. Also results showed that yield of combined application of 80% conventional fertilizer of nitrogen and nano-carbon at 0.6%was equal to that with supplied 100% conventional fertilizer (control). This indicated that the utilization rate of nitrogen fertilizer was increased after combined application of nano-carbon, which can save the N fertilizer amounts in production practice.